8. January 2019

Elementar Data Bulletin

CHNS analysis in liquid fuels with thevario EL cube.

The CHNS content of liquid fuels is usually determined as an indication for its quality and fuel value. Liquid fuel samples filled in capsules can be analyzed with the vario EL cube in its standard set-up.

The samples are weighed into tin capsules which are flushed with O2 while
sealing the capsules, in order to avoid ambient air inclusions. The daily factor has been determined using stearic acid and sulfanilic acid.

Click here to view Data Bulletin

Read more

8. January 2019

Rigaku EDXRF Application Note # 1856

Additive elements in lubricating oils.

Scope
Measurement of additive elements P, S, Cl, Ca, Zn and Mo in lubricating oils by ASTM D7751 is demonstrated.

Backgound
Controlling additive elements when blending new, fresh lubricating oils is vital to ensure correct lubrication properties and minimizing production waste and product rejection. EDXRF is a fast and simple analytical technique for use by non-technical operators, yet powerful enough for expert use in the research & development of new lube oil formulations. To meet the industry needs in production and research Rigaku NEX DE offers compliance to D7751 Standard Test Method for Additive Elements in Lubricating Oils by EDXRF Analysis for P, S, Cl, Ca, Zn and Mo.

Click here to view Application Note

Read more

8. January 2019

Rigaku Application Note #1082

Sulfur Analysis in Crude Oil and High-Sulfur Fuels by Benchtop WDXRF.

Introduction
Crude oil is raw material for petroleum products and contains sulfur in concentration from 0.5 mass% to 5.0 mass% typically. Residual fuel oil and high-sulfur diesel fuel are mainly used for boilers and burners, agricultural machines, and long-distance mass transportation means, such as vessels and diesel locomotives of rail road, equipped with large engines. Sulfur compounds in petroleum cause various harmful

influences such as air pollution, metal corrosion and catalyst degradation. Therefore, sulfur concentration of crude oil and high-sulfur fuels is monitored or controlled in refinery and production processes in the petroleum industry.

Read the full Application Note

Read more

26. November 2018

Video: The Cytoflex Flow Cytometer

Curious about the Cytoflex platform from Beckman Coutler? View this video guide.

Read more

12. November 2018

Rapid Generation of High- Producing Clonal Cell Lines

In this article the Janssen R&D cell-line development (CLD) group partnered with Sphere Fluidics to evaluate the Cyto-Mine system.

The Janssen R&D cell-line development (CLD) group partnered with Sphere Fluidics to evaluate its Cyto-Mine system: a high-throughput microfluidics instrument developed to address unmet needs in the monoclonality and productivity screening stages of cell-line development processes. The instrument takes a mixed population of cells loaded by a user and encapsulates individual cells into picodroplets.

The machine then analyzes each individual droplet for productivity using a fluorescence resonance energy transfer (FRET)–based screen, then images and dispenses the highestproductivity droplets into individual microplate wells. The entire process occurs inside a singleuse cartridge with media and reagents that are completely free of animal-sourced components.

Read the full article here

Read more

12. November 2018

Video: Cyto-Mine® Operation guide

This video gives an overview of the use and workflow of the Sphere Fluidics Cyto-Mine single cell analysis system.

Read more

12. November 2018

Cyto-Mine WebEx session

Learn more about the powerful system from Sphere Fluidics here.

Are you looking to accelerate your cell line development?
The CytoMine® can help!

The CytoMine® Sphere Fluidics can simplify your workflow dramatically by providing a high-throughput cell analysis assurance system without compromising on cell integrity and accuracy.

Learn more about the powerful system on a webex:
November 22 at 14:00 CET
November 23 at 12:00 CET
 
Sign up by sending a email to: LAS@Ramcon.dk
Read more

12. November 2018

Centrifuge 101 - Knowledge is power

The majority of all centrifuge accidents that happen in today's lab result from user error. new videos from Science Insider.

The majority of all centrifuge accidents that happen in today's lab result from user error. Safe and correct use of centrifuges, rotors and accompanying labware will help prevent rotor failure and any other centrifuge related accidents, keeping labs to run efficiently.

Centrifuges may operate at very high speeds, but when deconstructed, are simple machines comprising of a rotor, a drive shaft and a motor. To know and understand the principles of centrifugation gives you the power to run a smooth and efficient research, diagnostic or clinical lab. Below are some of our resources we have packaged to make it easy to train your operators.

 

3 videos from Science Insider explains centrifugation and what it means in today's lab, and in depth how centrifuges, rotors and the associated labware operates.

Have a look at the videos here

Read more

24. October 2018

SCIEX C100HT Application

Automating the C100ht Biologics Analyzer Sample Preparation on a Biomek i5 Liquid Handler Platform.

Introduktion

There is an increasing need in the biopharmaceutical industry for large-scale N-glycosylation profiling of therapeutic antibodies in all phases of product development. This need is highlighted particularly during clone selection and cell culture optimization where hundreds of samples should be analyzed in a short period of time to properly assess their glycosylation profiles.

The SCIEX C100ht Biologics Analyzer is a high throughput glycan screening platform consisting of a pre-filled multi-capillary cartridge enabling end users to process hundreds of samples per day.1 C100ht glycan sample preparation (digestion, labeling and clean up) is a magnetic bead—mediated process eliminating lengthy, manual centrifugation and vacuum—centrifugation steps found in traditional protocols.
This technical note describes the steps necessary for automation of C100ht sample preparation workflow using MAK33, a purified recombinant monoclonal antibody.

Key Features

  • Reproducible high throughput screening of glycans
  • Automated sample preparation
  • Step by step automation instructions
     

Read the full application here

Read more

22. October 2018

Centrifuge HarvestLine system

HarvestLine system Liners for Avanti high performance centrifuges from Beckman Coulter,

Labware is only the beginning when it comes to obtaining results in the lab. Safe, efficient workflows are as critical to your work as quality samples.  Now you can harvest high-quality samples in fewer steps from prep to cleanup with Beckman Coulter's HarvestLine System.  

Using the HarvestLine System in your lab removes the need for non-value-added centrifugation steps such as extra spins, bottle cleaning, bottle scraping, and added time for freeze/thaw lysis. In addition, the medical-grade polyurethane material is flexible and capable of storing samples more efficiently. Liners are available in sterile and non-sterile.

Ideal applications for Beckman Coulter bottle liners include:  Harvesting bacterial and yeast cultures, Lysate clarification, isolation or recovery of vaccine components, cell and debris sedimentation, microparticle pelleting, protein separations, bacterial cell pelleting, and clarification of tissue homogenates.

In the table below, select the HarvestLine kit compatible with your instrument and rotor. Refer to product pages for kit contents.

 

Click here to learn more

Read more

9. October 2018

Journal from Rigaku

Investigation for fuel-cell structures with multi-scale X-ray analysis.

Introduction
It is well known that X-rays have wavelengths comparable with interatomic distances and can be utilized for atomic-scale structural determination. In addition, X-rays can penetrate through opaque objects and show the internal structure without destroying the object. Recently, the resolution of X-ray microscopes has been pushed down to the sub-micrometer level. These devices are also capable of recording computed tomography (CT) data. This is a powerful technique for visualizing the internal structure of various specimens in three dimensions (3D) at the micrometer scale.

Read the full journal here

Read more

9. October 2018

Video: Updated SmartLab from Rigaku

SmartLab is the ideal tool for powder- and thin film diffraction, stress- and texture measurements, SAXS, in-plane scattering and in-situ measurements.

Rigaku released the updated SmartLab earlier this year. SmartLab is the ideal tool for powder- and thin film diffraction, stress- and texture measurements, SAXS, in-plane scattering and in-situ measurements. Check out the functions of the SmartLab in this video

Watch the video below:

Read more

9. October 2018

Nikon CT Case story

The metal additive manufacturing company Sintavia adds Nikon Metrology CT to its state-ofthe-art facility in Florida, USA.

Sintavia gets the inside track with micro-CT

Additive manufacturing is making enormous waves throughout every industry. The technology allows ultimate flexibility in creating the cost intricate and complex features ever designed for manufacturing. 

As Sintavia sets itself apart with its beginning-to-end manufacturing, its biggest asset is having full and total control of the additive manufacturing process. Without complete control, you do not have complete understanding of the process.

Read the case story here

Read more

27. September 2018

Elementar Data Bulletin

Organic, inorganic and elemental carbon analysis in soil using the soli TOC cube.

The analysis of the different fractions of carbon in soil is an important tool for assessing soil quality. The new soli TOC cube offers users the option of measuring not only total organic and total inorganic carbon, but also determining the total elemental carbon (ROC) using a temperature ramping program. This is important, because elemental carbon is not bioavailable.

View the full PDF here

Read more

27. September 2018

EDXRF Application Note #1385

The analysis of agricultural soils and plant materials is demonstrated.

BACKGROUND
In the agri-food sector it is important to not only study the soil composition and use of fertilizers, but also the uptake of nutrients and potentially toxic elements within the plants and crops themselves. XRF (X-ray Fluorescence) is an accepted technique in the industry. 

Download the application here

Read more

27. September 2018

EDXRF Application Note #1105

Trace elements in aqueous solution with ultracarry.

SCOPE
Elemental analysis of aqueous solutions into the low ppm and sub-ppm concentration ranges was demonstrated, using the advanced Cartesian geometry Rigaku NEX CG Energy Dispersive X-ray Fluorescence (EDXRF) spectrometer in conjunction with the patented UltraCarry® sample preparation technique.

Download the Application note here

Read more

14. September 2018

LIPSI from Nikon is here!

High-speed, flexible, automated well plate acquisition, data visualization and analysis.

Nikon Europe has developed, in collaboration with key players in the field Prior scientific and Life Imaging Services, a fully incubated, high content screening platform that enables high speed acquisition for up to 20 well plates. Together with our analysis tools, Nikon enables you to get results from live cells in real time.

LIPSI utilises all the functionality of the Eclipse Ti-2 inverted microscope, including its 25mm field of view and capacity of high speed imaging, while offering modularity and expandability. LIPSI can easily be upgraded with different confocal modalities and accessories to ensure that your platform can evolve with your research needs.

Click here to learn more

Read more

14. September 2018

New Multiwell plates from ibidi

Multiwell plates with black walls and a flat and clear coverslip bottom.

Need High Throughput for your Cell Culture Assays?

  • High-resolution cell microscopy on a tissue culture-treated surface
  • Brilliant Fluorescence imaging without autofluorescence 
  • Ready for HTS and robotics: compliant with ANSI / SLAS (SBS)

Multiwell plates with black walls and a flat and clear coverslip bottom (#1.5 ibidi Polymer Coverslip) for high-throughput applications in cell-based assays.

Click for more info and FREE samples

Read more

3. September 2018

New CytoFLEX poster

27-Marker High-Sensitivity Experiments on a 3-Laser CytoFLEX.

While a small number of channels, and thus detectors, on a flow cytometer can be a limitation, a large number does not necessarily confer a great advantage: the maximum number of biomarkers that can be analyzed by a flow cytometer is not actually limited to the number of channels. The number of markers that can be used with a conventional flow cytometer can be increased by simply stacking biomarkers that do not co-label the populations of interest. In this poster, Beckman Coulter demonstrates how advanced users of flow cytometry may overcome the limitation of channels on a flow cytometer.

View the poster here

Read more

23. August 2018

Chosing the right Agencourt reagent kit

Review the reagent chart from Beckman Coulter to determine the optimal Agencourt reagent kit for your workflow.

No matter how advanced the technology becomes, sequencing results are always impacted by sample quality. It's important to optimize every, equally-important step of your sample prep workflow starting with nucleic acid extraction. Do you want to get more data out of your extraction? Are you looking to evaluate better, scalable reagent solutions? Review the chart below to determine which Agencourt reagent kit can best support your workflow based on your extraction needs and starting samples.

View the reagent chart here

Read more

23. August 2018

New Agencourt FormaPure reagent kit

A single chemistry system designed and optimized for use in Next-Generation Sequencing (NGS).

Nucleic acid extraction from formalin-fixed, paraffin-embedded (FFPE) tissue is challenging due to the nature of the tissue preparation. Agencourt FormaPure reagent kits represent a single chemistry system designed and optimized for use in Next-Generation Sequencing (NGS) as well as other downstream applications including qPCR/ddPCR/PCR. Maximizing integrity, yield and purity from an FFPE sample is required to minimize the risk of losing important genetic information.

View the full Formapure datasheet here

 

Read more

23. June 2018

When to replace a centrifuge?

Lab Manager Centrifuge resource guide.

Lab Manager has published a video guide on what to look out for on your centrifuge, and when it might time for a replacement.

 

 

 


 

The film can be viewed here:

Along side this, Lab Manager provides a guide on centrifuges that covers the following topics:

  • Questions to ask when purchasing a new centrifuge 
  • Maintenance tips to keep your centrifuge and rotors spinning safely.
  • Expert advice on set-up, care, and techniques
  • Featured products and new offerings

Download the guide here

Read more

21. June 2018

CAR-T workflow

Manufacturing Processes for Engineered T-Cell Therapy – CAR-T using Beckman Coulter instruments.

Manufacturing Processes for Engineered T-Cell Therapy – CAR-T

Why T-cells are important

  • They recognize host vs. non-host cells to initiate an immune response
  • They're the primary immune system agents that attack diseased cells or infection
  • They're used in several cell-based therapies:

Transplantation of donor T-cells - a bone marrow
transplant, in which a matched donor's cells are used
to replace the patient's cells, is the most widely used
approach—typically for diseases that originate in the
immune system (e.g., leukemia)

Isolation and enrichment of a patient's T-cells – an
approach considered the safest because it enhances
a patient's own immune response to disease

CAR-T therapy – a more recent approach in which
T-cells are removed, genetically engineered to express
a synthetic receptor against specific cancer cells, then
infused back into the patient's bloodstream; this therapy
is effective because many cancer cells "hide" from the
immune system by appearing to be normal cells, thereby
triggering no immune response

View the full workflow here

Read more

21. June 2018

SelectScience CAR-T article

Advances in CAR T Therapy: Reprogramming Patient Cells to Treat Cancer.

Learn about the six steps of CAR T cell production and the technology ensuring optimal cell delivery to patients


Chimeric antigen receptor modified T cell (CAR T) therapy is an emerging immunotherapy that involves the genetic engineering and ‘supercharging' of patients' own immune cells to treat their cancer. In this SelectScience® interview, Dr. Richard Jones, Senior Manager of Global Marketing, Particle Counting & Characterization Group, Beckman Coulter, describes the latest advances in CAR T therapy and outlines the important role that Beckman Coulter's Multisizer cell counting and volume analysis technology has in the delivery of this novel treatment to patients.

Click here to read full article from SelectScience

Read more

15. June 2018

Preparation and XRF Analysis of Fly Ash Fused Beads

Application Note from Spex Sample Prep.

Fly ash is a waste product from the combustion of coal and is comprised of the mineral particles that rise with the flue gasses. Recovered fly ash is used as component in certain cement mixes and improves the durability and strength of concrete.

In this study, a sample of fly ash was pulverized and blended, and then fused into two sets of glass beads using a Katanax K1 electric fusion fluxer. Two different lithium borate fusion fluxes from SPEX CertiPrep were used to prepare five beads with each flux. The beads were then analyzed by energy dispersive X-ray flourescence spectroscopy (ED-XRF) and the results were compared both within a set and between the two sets to evaluate consistency of beads produced using the K1 and effect of flux composition on the XRF measurements.

View the Application Note here

Read more

15. June 2018

Application from Elementar using the UNICUBE

CHNS analysis of substances with extraordinary elemental composition.

Some substances carry extraordinary elemental ratios. A prerequisite for the elemental analysis of such compounds is reproducible analytical performance of the instrument and rigorous matrix independence of the measurement. This in turn requires quantitative conversion of the sample into the gas phase through full combustion, as well as a gas separation system that is free of matrix effects – always and in all circumstances. The UNICUBE® unifies all these requirements.

View the Application 

Read more

15. June 2018

Rigaku Application Note

#1705 - The measurement of trace levels of Al, Si, P, Cl, Ti and Ca in polypropylene is demonstrated.

Background
In the production of polymers such as polypropylene (PP), polyethylene (PE) and polyethylene terephthalate (PET, PETE) various additives and stabilizers are added, as well as Si (as SiO2) as an anti-block. Trace levels of catalyst residues may also be present. To ensure proper quality control during the production process test samples are made and the trace elements are measured. To meet this industry need Applied Rigaku Technologies offers a full line of benchtop EDXRF analyzers. With simple, intuitive software interface and powerful, innovative design, Rigaku EDXRF systems make an excellent tool for quality control check at several places along the polymer production process.

View the Application Note here

Read more

8. June 2018

Application Note from ibidi

A web-based quantitative image analysis solution for wound healing and cell migration assays.

A web-based quantitative image analysis solution for wound healing and cell migration assays.

  • A complete solution for wound healing experiments – only a few steps from sample preparation to image analysis
  • Objective and reproducible analysis
  • Easy and fast data processing – results within minutes
  • No extra hardware or software needed

Click here to read the Application

Read more

8. June 2018

Wound Healing App Note from Biotek

Wound Healing monitoring using the Lionheart™ FX Automated Live Cell Imager.

Performance of a Label-Free Image-Based 2D Scratch Wound Healing Assay to monitor Cell Migration and its Inhibition.

Introduction
Cell migration- the random movement of cells plays a central role in physiological and pathological processes. Cell migration is an integral part of basic biological processes like tissue formation, wound healing and the immune response, as well as processes like cancer invasion and metastasis.

The basic principal of cell movement is a cyclical 5-step procedure starting with cell polarization and protrusion of the leading edge.

Read the Application Note here

Read more

31. May 2018

Western blot technique

Western blot is a well-established and widely used technique for the detection and analysis of proteins.

Western blot is a well-established and widely used technique for the detection and analysis of proteins. The method is based on the construction of an antibody-protein complex with specific antibodies that bind to proteins immobilized on a membrane. In the last decade, The Fusion has permit great improvements in detection methods and software that have made quantitative Western blot safer and more sensitive and efficient.

Read the article on Western Blotting detection methods from Vilber here

Read more

24. May 2018

Avanti JXN Series - Complete protein purification workflow

Centrifuge application note from Beckman Coulter.

Purified proteins are required for many proteomics applications such as X-ray crystallography, NMR, mass spec, and in vitro biochemical assays. Proteins can be isolated from tissue or, more often, by their overexpression
in an organism, like bacteria, yeast, or mammalian cells in culture. Characteristics unique to each protein, like amino acid composition, size, shape, isoelectric point, and solubility are used to develop unique strategies
for isolation of the protein of interest. The objective is to isolate the largest amount of functional protein of interest with the least amount of other contaminants present.

View the application here

Read more

10. April 2018

The LS 13 320 XR is here!

New LS 13 320 XR Laser diffraction particle size analyzer from Beckman Coulter.

The LS 13 320 XR boosts laser diffraction particle size analysis to the next level, with its enhanced PIDS technology and extended measurement range providing higher resolution and more accurate, reproducible results. You can measure a wider range of particles and detect smaller differences in samples more quickly and reliably. And new software with an intuitive interface provides data you need with only a few clicks.

Click here for product information

Download brochure

Read more

6. April 2018

CD133-APC - New single Color Antibody

New Antibody released from Beckman Coulter.

The CD133 single color reagent from Beckman Coulter is a key marker in the field of hematopoietic stem cells, being the first CD marker expressed on early hematopoietic stem cells, before any lineage antigen. 

Click here to download data sheet

Read more

19. March 2018

EDXRF Application Note From Rigaku

The measurement of Mg, Si, P, S, Cl, Ca, Zn, Ba and Mo in new lube oils is demonstrated using the empirical approach.

Quality control and quality assurance during the lube oil blending and manufacturing process is essential. Basic lube oil formulations containing only P, S, Ca and Zn are often augmented with additional elements and additives to create lubricating oils for specific needs. A fast, simple method of analyzing lube oils is important throughout the QC/QA process. Rigaku meets this industry need with a high performance low cost benchtop EDXRF system.

Download the Application Note here

Read more

19. March 2018

Application from Rigaku

Ultra Low P, S, Cl Analysis in Liquids with the Supermini200 Benchtop WDXRF Spectrometer

Phosphorous, sulfur and chlorine are commonly analyzed elements in liquids such as petroleum products and organic solvents. For example, phosphorous is added to lubricating oils to prevent wear of gears under high pressure.

To better meet demands of such applications, the benchtop wavelength dispersive X-ray fluorescent (WDXRF) spectrometer Supermini200's analyzing crystal set has been newly reconfigured to optimize performance for trace level analysis of P, S and Cl.

Download the application note here

Read more

19. March 2018

Application Note From Elementar

Determination of low sulfur concentrations in mineral oil using the trace SN cube.

The determination of sulfur in diesel, biodiesel, diesel/biodiesel blends, and jet fuel is a very important measure, because too high concentrations of sulfur can be extremely degrading to engine components. Consequently for such purposes, a high performance analysis is required.

Download the application note here

Read more

15. March 2018

Application from BMG LABTECH

Cellular and biochemical HTRF® assays measured in 1536-well microplates.

Screening for new candidate drugs is a standard procedure in the pharmaceutical industry. However, there are increasing requirements on assays and instrumentation. Especially the number of samples measured per day should be further increased.

 

 

This can be realized if high condensed microplates are used, e.g. 1536-well microplates. The instrument of choice must be able to measure such microplates with the fastest possible read time while the assay performance criteria (such as Z´value or S/B) shall be reached. To meet the requirements for ultra high throughput screening BMG LABTECH has developed the microplate reader PHERAstar FSX. The new TRF laser enables fastest read times in 1536-well microplates. Two HTRF assays from Cisbio Bioassays were selected to highlight the enhanced performances achieved with the PHERAstar FSX.

Read the application note from BMG LABTECH here 

Read more

9. February 2018

How to prepare particle dispersion in liquid

In particle characterization, particles in a sample have to be dispersed in a medium in order to be characterized.

Introduction
In particle characterization, particles in a sample have to be dispersed in a medium in order to be characterized. Liquid sample dispersion is when we attempt to suspend a dry powder in an aqueous or non-aqueous liquid medium before analysis. The particular characteristics of the dry powder dictate what medium should be utilized as a diluent. Factors such as solubility, reactivity, suspendibility and end use govern the choice of the medium.

Read the full article here

Read more

8. January 2018

DNA quantification with BMG LABTECH

UV absorbance or fluorescence DNA measurements with BMG LABTECH plate readers.

Accurately quantitate nucleic acids on BMG LABTECH instruments

The quantification of nucleic acids (DNA or RNA) in samples is part of the daily routine in laboratories.  the fluorescent quantification of DNA and RNA has become quite popular. With this method, an increased fluorescence signal is measured once the dye has bound to nucleic acids. 

Click here for more information

Read more

28. November 2017

Application from Elementar

Carbon and sulfur analysis in metal powders for 3D printing.

More and more metallic parts are produced via 3D printing. This new technology offers completely new opportunities regarding complex geometries, which have never before been thinkable to produce. 

However, after multiple uses the carbon and sulfur concentrations and the related mechanical properties can be altered. Therefore, it is important to monitor the carbon and sulfur concentrations of the metal powders in order to ensure high quality, 3D printed parts.

Download the full document here

Read more

28. November 2017

Application from Elementar

Determination of organic and inorganic carbon impurities in fluorite.

Fluorite (CaF2) is an important industrial mineral. It is widely used in the metal, chemical, ceramic, glass, and optical industry. Carbon is, among other elements, one of the common impurities found in fluorite. With the soli TOC cube it is possible to determine the amount of organic and inorganic carbon in fluorite in a single measurement.

Read the full document here

Read more

28. November 2017

Application from Elementar

Accuracy of TOC concentrations in mineral water measured with the acquray TOC.

OVERVIEW
Excellent accuracy for TOC analyses in mineral water with standard addition using the acquray TOC.

Besides a high precision, which is given by the acquray TOC, the accuracy of the results is of great importance for analytical purposes. The method of standard addition with mineral water was used to provide proof that the acquray TOC is a perfect solution for TOC analysis in drinking water.

View the full document here

Read more

24. November 2017

Chemotaxis webinar from ibidi

Webinar: In vitro Monitoring of the Intrinsic Motility Behaviors of Human Lymphocytes. 20 Nov 2017

Webinar outline
The motility of lymphocytes is intimately related to their immune surveillance function. Lymphocytes alternate between phases of individual cell exploration across tissues and phases of prolonged cell-cell interaction during activation and function. We are exploring how diversified lymphocyte motility behaviors emerge from the capacity of those cells to integrate signals from their environment, including extracellular matrix components, chemokines, neighbor cells and antigen-presenting cells.

 

Time and date:
November 29 2017 at 10:00 and again at 16:00 (Copenhagen timezone)

Click here for registration 

Read more

24. November 2017

Workflow Challenges Solved: Chr. Hansen

Video: Our customer Chr. Hansen explains how liquid handling automation helped them become more productive in the lab.

Our customer Chr. Hansen explains the workflow challenges they faced and how liquid handling automation helped them become more productive in the lab.

 

 

 



Watch the video here:

Read more

31. October 2017

Quantitation Insights - eBook from SCIEX

How to approach therapeutic quantitation in more innovative and productive ways.

Explore how to approach therapeutic quantitation in more innovative and productive ways with high-performance hardware, software, sample prep and lab-tested analysis methods. Packed with technical articles on a range of applications and techniques, this eBook will help you to discover how you can transform your quantitation workflows.

Download the eBook here

Read more

16. October 2017

Nikon Small World Winners

View all the amazing microscopy images from the competition here.

The Nikon Small World Photomicrography Competition celebrates the beautiful, weird, wonderful, and microscopic things in our world through photos captured using a light microscope. Now in its 43rd year, the competition continues to impress.

The 2017 competition received over 2,000 entries from 88 different countries. Many of the images were taken during scientific research projects.

"This year's winners not only reflect remarkable research and trends in science, but they also allow the public to get a glimpse of a hidden world," says Eric Flem, Communications Manager, Nikon Instruments.

Have a look at all the amazing images here

Read more

5. October 2017

Sulfur analysis in rubber

Application from Elementar - Determining hardness degree of vulcanized rubber.

With elemental analysis, it is possible to determine the exact hardness degree of vulcanized rubber for which the sulfur proportion is essential. Elastomers that contain sulfur cross-linkages by vulcanization ensure high fatigue resistance and a low wear rate for rubber of all types that are exposed to mechanical stress.

To determine the sulfur concentration in rubber, a sample is combusted with the help of catalyst oxygen. The released sulfur dioxide is measured by an integrated thermal conductivity detector and measured as a percentage. With the Elementar analyzer, it is possible to provide high data quality for a variety of substances relevant to the production, or recycling, of rubber products.

 

View the full document here

Read more

1. October 2017

New Ebook from Nikon

‘Sharper images: Exploring confocal and superresolution microscopy' -New Ebook from Nikon.

Sharper images: Exploring confocal and superresolution microscopy' -from Science and Nikon Instruments Inc.

 

 

 

 


This ebook is a collection of research articles highlighting the application of superresolution and confocal imaging technologies. In addition, a technical paper reviewing the latest developments in Nikon confocal technology and an application note comparing the benefits and drawbacks of superresolution versus confocal systems are included. We hope this booklet provides a timely review of innovations and applications in both the confocal and superresolution technology space, and aids the reader in selecting the best imaging technology for their research.

 

Download the Ebook here

Read more

14. June 2017

Advanced analysis of human T cell subsets on the CytoFLEX

Application Information Bulletin: 13 colors on CytoFLEX.

Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube based on DuraClone dry reagent technology.

 

 

 

 

Summary
Multi-color flow cytometry is a powerful tool to analyze the highly heterogeneous human T cell compartment.
Using the 10 color DuraClone IM T Cell Subset dry reagent kit (CD45RA-FITC, CD197-PE, CD28-ECD,
CD279-PC5.5, CD27-PC7, CD4-APC, CD8-Alexa-Fluor* 700, CD3-APC-Alexa-Fluor* 750, CD57-Pacific Blue*,
CD45-Krome Orange) plus 3 additional liquid antibodies for the violet laser (Brilliant Violet 605TM anti-human
CD95, Brilliant Violet 650TM anti-human CD25, and Brilliant Violet 785TM anti-human CD127 antibodies) we
defined a 13-color tube which allows for the identification of major peripheral T cell subsets according to
classical and more recent characterization criteria, with a minimum of sample preparation effort.

 

Download the full application here

Read more

14. March 2017

Analysis of Animal Feeds

Quality control of raw materials, ingredients, mix or finished with X-ray fluorescence is now faster and better than ever.

Quality control of raw materials, ingredients, mix or finished with X-ray fluorescence is now faster and better than ever.

Determination of Na, Mg, P, Cl, K, Ca, Mn, Fe and many more, with detection limits of 1 ppm - measured directly, without significant sample preparation.

Thanks to the rapid development of detectors, optics and with Rigaku's RFP-SQX Shortfin and flying software, measured all the items at once and without calibration.

Read the "Analysis of Animal Feeds" application from Rigaku here.

 

Read more

16. February 2017

Fast glycan labeling & analysis

High Resolution Glycan Analysis in Record Time. New kit from SCIEX.

SCIEX Fast Glycan Labeling and Analysis delivers rapid glycan heterogeneity identification, capable of profiling your glycans in record time. Combining simplified sample preparation, rapid separations and automated glycan identification, you can make confident decisions quickly by running glycans up to five-times faster than HILIC.

 

 

 

 

Up to 5x Faster Than HILIC

SCIEX Fast Glycan technology enables you to rapidly perform glycan separation while retaining high resolution information. This means you can characterize more samples in less time for quicker comparability assessments across a population of samples. You can also minimize the cycle time needed to course correct processes, from development to commercial manufacturing.

Automated and Immediate Glycan Identification

The comprehensive GU based glycan reference database delivers fast separations with automated and immediate identification. Key glycan GU values are established using the SCIEX Fast Glycan assay and offer efficient identification - reducing time and minimizing errors - without the need to use technologies requiring advanced expertise.

Sample Preparation Made Easy

Based on award winning magnetic-bead technology, the SCIEX Fast Glycan assay does not require ultra-centrifugation or advanced pipetting techniques. This makes the assay suitable for manual pipetting as well as automation. The short incubation times, combined with magnetic-bead based sample clean-up, allows you to complete sample prep in an hour. Furthermore, the magnetic-bead reduces the presence of unbound dye resulting in better characterization of complex sialylated structures.

 

Click here for more information

 

Read more

10. February 2017

Stony-iron meteorite analysis

Analysis of a stony-iron meteorite using a HyPix-3000 multi-dimensional detector.

Introduction
A stony-iron meteorite is like a single crystal. When using 0- or 1-dimensional detectors, only a few diffraction peaks are observed. In this situation, identification of crystalline phases cannot be conducted. Therefore, a stony-iron meteorite was analyzed using the multi-dimensional detector, HyPix-3000, which has an effective detection area for the identification of crystalline phases.

 

 

Measurements and results
A stony-iron meteorite was analyzed using the HyPix-3000. Inside a stony-iron meteorite, there are transparent parts similar to glass and opaque parts similar to metal. The transparent parts were thought to be non-crystalline (amorphous). However, when measurement of the transparent part was actually performed, only one diffraction line was observed. In general, when measurement is done with a 0D or a 1D detector, the range in which diffraction X-rays can be detected from the sample is limited to a certain region. (This region is indicated in yellow in Fig. 1.) In this experiment, the diffraction lines, indicated with red arrows, are within the scanning range, and therefore can be detected. On the other hand, it is possible to acquire information on multiple lattice planes, as shown in Fig. 2, by using the HyPix-3000, which has a wide effective detection area, and by performing measurement while oscillation the sample. When the 2D diffraction image obtained in Fig. 2 was converted to a 2θ-I profile and qualitative analysis of the transparent part was carried out, it was possible to identify forsterite (Mg₂SiO₄) (Fig. 3). Since diffraction spots were observed, it was conjectured that the glass-like transparent part is a single crystal or is comprised of an extremely small number of crystal grains.

 

 

 

 

 

 

Fig. 1: X-ray diffraction pattern of glass-like transparent part when using a 0D or 1D detector

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Fig. 2: 2D diffraction image of transparent part (with oscillation)

 


Fig. 3: Result of qualitative analysis of transparent part


Recommended equipment and software

  • Automated Multipurpose X-ray Diffractometer SmartLab
  • 2D Hybrid Pixel Array Detector HyPix-3000

 

 

 

 

 

 

 

 

 

The SmartLab is the most novel high-resolution diffractometer available today.  
Perhaps its most novel feature is the SmartLab Guidance software,
which provides the user with an intelligent interface that guides you through the intricacies of each experiment. It is like having an expert standing by your side.
For more information click here.

Read more

12. January 2017

Immunoflourescence Assays

Removable Chambers or All-in-One Chambers for Immunofluorescence Assays.

 


 

 

 

 

 

Classic Immunofluorescence in Removable Chambers

ibidi provides the easiest and most convenient solution for removable culture wells. These products contain a silicone gasket with chambers for individual cell culture and incubation conditions. The Immunofluorescence Chamber Family is perfect for long-term storage of samples prepared using the classic "glass slide with mounted coverslip" technique.


Immunofluorescence in All-in-One Chambers

ibidi provides a time saving and cost-effective solution for immunofluores¬cence with ibidi µ-Slides, an All-in-One chamber. Choose between the traditional open-well chambers or the channel µ-Slides. The channel slides are ideal for reducing the needed volume of expensive reagents or staining antibodies.  Both formats allow cultivation, fixation, permeabilization, and staining steps to be completed in the wells or channels.  After staining and mounting, the sample can be observed through the coverslip-like bottom by high-resolution microscopy.

 

Find more information how to optimize your immunofluorescence protocol with these application notes:

Removable Chambers:
Application Note 49: Immunofluorescence Staining with the 12 Well Chamber, removable
Application Note 50: Immunofluorescence Staining with the 3 Well Chamber, removable
Application Note 45: Mounting Medium Types for Immunofluorescence Microscopy

All-in-One Chambers
Application Note 16: Immunofluorescence Staining with the µ-Slide 8 Well
Application Note 09: Immunofluorescence Staining with the µ-Slide VI 0.4

Interested in testing how the slides work? 
Order Your FREE Sample
for Your Immunofluorescence Assay.

Ordering information:
µ-Slide 2 Well, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
µ-Slide 4 Well, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
µ-Slide 8 Well, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
µ-Slide VI 0.4, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
3 Well Chamber, removable: microscopy glass slide, sterilized
8 Well Chamber, removable: microscopy glass slide, sterilized
12 Well Chamber, removable: microscopy glass slide, sterilized

 

Read more

Contact us