14. juni 2018
Preparation and XRF Analysis of Fly Ash Fused Beads
Application Note from Spex Sample Prep.
Fly ash is a waste product from the combustion of coal and is comprised of the mineral particles that rise with the flue gasses. Recovered fly ash is used as component in certain cement mixes and improves the durability and strength of concrete.
In this study, a sample of fly ash was pulverized and blended, and then fused into two sets of glass beads using a Katanax K1 electric fusion fluxer. Two different lithium borate fusion fluxes from SPEX CertiPrep were used to prepare five beads with each flux. The beads were then analyzed by energy dispersive X-ray flourescence spectroscopy (ED-XRF) and the results were compared both within a set and between the two sets to evaluate consistency of beads produced using the K1 and effect of flux composition on the XRF measurements.
14. juni 2018
Rigaku Application Note
#1705 - The measurement of trace levels of Al, Si, P, Cl, Ti and Ca in polypropylene is demonstrated.
In the production of polymers such as polypropylene (PP), polyethylene (PE) and polyethylene terephthalate (PET, PETE) various additives and stabilizers are added, as well as Si (as SiO2) as an anti-block. Trace levels of catalyst residues may also be present. To ensure proper quality control during the production process test samples are made and the trace elements are measured. To meet this industry need Applied Rigaku Technologies offers a full line of benchtop EDXRF analyzers. With simple, intuitive software interface and powerful, innovative design, Rigaku EDXRF systems make an excellent tool for quality control check at several places along the polymer production process.
12. december 2017
Centrifuge 101 - Knowledge is power
Hovedparten af uheld i laboratorier i dag, skyldes brugerfejl. Disse tre enkle vidoer forklarer pricipper og korrekt brug af centrifugering.
The majority of all centrifuge accidents that happen in in today's lab result from user error. Safe and correct use of centrifuges, rotors and accompanying labware will help prevent rotor failure and any other centrifuge related accidents, keeping labs to run efficiently.
Centrifuges may operate at very high speeds, but when deconstructed, are simple machines comprising of a rotor, a drive shaft and a motor. To know and understand the principles of centrifugation gives you the power to run a smooth and efficient research, diagnostic or clinical lab. Below are some of our resources we have packaged to make it easy to train your operators.
3 videos from Science Insider explains centrifugation and what it means in today's lab, and in depth how centrifuges, rotors and the associated labware operates.
31. oktober 2017
Kvantitativ indsigt - Ebog fra SCIEX
Terapeutisk kvantificering på mere innovative og produktive måder.
Udforsk, hvordan du nærmer dig terapeutisk kvantificering på mere innovative og produktive måder med højtydende hardware, software, prøvepræparater og laboratorietestede analysemetoder. Denne Ebog fra SCIEX er pakket med tekniske artikler om en række anvendelser og teknikker, og hjælper dig til at udforske, hvordan du kan omforme dine kvanteringsarbejdsprocesser.
16. oktober 2017
Nikon Small World 2017 vindere
Se alle de fantastiske billeder fra Nikon Small World 2017 her.
Nikon har nu offentliggjort vinderne af 2017 udgaven af deres 2017 Small World Photomicrography Competition. Konkurrencen udbyder hvert år forskere og fotografer til at indsende billeder der taget er taget med mikroskoper. I år var den 43. udgave af konkurrencen og flere end 2000 billeder fra 88 lande deltog.
1. oktober 2017
Ny E-bog fra Nikon
Sharper images: Exploring confocal and superresolution microscopy - Ny E-bog fra Nikon.
"Sharper images: Exploring confocal and superresolution microscopy" frar Science og Nikon Instruments Inc.
Denne E-bog er et kompendie af research artikler med fokus på applikation af superresolution og konfokale billedteknologier. Derudover et mere teknisk dokument der rummer et overblik over den seneste udvikling indenfor Nikon konfokal teknologi, samt en applikation note der sammenligner fordele og ulemper ved superresolution sammenlignet med konfokal systemer.
23. juni 2017
Hvornår bør en centrifuge udskiftes?
Guide til centrifuger fra Lab Manager her.
Lab Manager har publiceret en film der giver et indblik i hvornår det kan være tid til at udskifte en centrifuge i et laboratorie.
Filmen kan ses herunder:
Derudover er der lavet en skreven guide omhandlende centrifuger, der dækker følgende emner:
- Spørgsmål omkring indkøb af ny centrifuge
- Vedligeholdelse af centrifuger
- Ekspertråd omkring opsætning og teknikker
- Aktuelle produkter
14. juni 2017
Advanced analysis of human T-cell subsets on the CytoFLEX
Application Information Bulletin: 13 colors on CytoFLEX
Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube based on DuraClone dry reagent technology.
Multi-color flow cytometry is a powerful tool to analyze the highly heterogeneous human T cell compartment.
Using the 10 color DuraClone IM T Cell Subset dry reagent kit (CD45RA-FITC, CD197-PE, CD28-ECD,
CD279-PC5.5, CD27-PC7, CD4-APC, CD8-Alexa-Fluor* 700, CD3-APC-Alexa-Fluor* 750, CD57-Pacific Blue*,
CD45-Krome Orange) plus 3 additional liquid antibodies for the violet laser (Brilliant Violet 605TM anti-human
CD95, Brilliant Violet 650TM anti-human CD25, and Brilliant Violet 785TM anti-human CD127 antibodies) we
defined a 13-color tube which allows for the identification of major peripheral T cell subsets according to
classical and more recent characterization criteria, with a minimum of sample preparation effort.
14. marts 2017
Lynhurtig kvalitetskontrol på foder
Kvalitetskontrol af råvarer, ingredienser, mix eller færdigvarer med Røntgen Flourescens er nu hurtigere og bedre end nogensinde.
Bestemmelse af Na, Mg, P, Cl, K, Ca, Mn, Fe og mange flere, med detektions grænser fra 1 ppm - måles direkte, uden nævneværdig prøve forberedelse.
Takket være den rivende udvikling af detektorer, optik og med Rigaku's RFP-SQX software, måles alle elementer på én gang og uden kalibrering.
16. februar 2017
Fast Glycan Labeling & Analysis
Nyt revolutionerende kit fra SCIEX, der henvender sig til alle CE-brugere.
SCIEX Fast Glycan Labeling and Analysis delivers rapid glycan heterogeneity identification, capable of profiling your glycans in record time. Combining simplified sample preparation, rapid separations and automated glycan identification, you can make confident decisions quickly by running glycans up to five-times faster than HILIC.
Up to 5x Faster Than HILIC
SCIEX Fast Glycan technology enables you to rapidly perform glycan separation while retaining high resolution information. This means you can characterize more samples in less time for quicker comparability assessments across a population of samples. You can also minimize the cycle time needed to course correct processes, from development to commercial manufacturing.
Automated and Immediate Glycan Identification
The comprehensive GU based glycan reference database delivers fast separations with automated and immediate identification. Key glycan GU values are established using the SCIEX Fast Glycan assay and offer efficient identification - reducing time and minimizing errors - without the need to use technologies requiring advanced expertise.
Sample Preparation Made Easy
Based on award winning magnetic-bead technology, the SCIEX Fast Glycan assay does not require ultra-centrifugation or advanced pipetting techniques. This makes the assay suitable for manual pipetting as well as automation. The short incubation times, combined with magnetic-bead based sample clean-up, allows you to complete sample prep in an hour. Furthermore, the magnetic-bead reduces the presence of unbound dye resulting in better characterization of complex sialylated structures.
10. februar 2017
Stony-iron meteorite analysis
Analysis of a stony-iron meteorite using a HyPix-3000 multi-dimensional detector.
A stony-iron meteorite is like a single crystal. When using 0- or 1-dimensional detectors, only a few diffraction peaks are observed. In this situation, identification of crystalline phases cannot be conducted. Therefore, a stony-iron meteorite was analyzed using the multi-dimensional detector, HyPix-3000, which has an effective detection area for the identification of crystalline phases.
Measurements and results
A stony-iron meteorite was analyzed using the HyPix-3000. Inside a stony-iron meteorite, there are transparent parts similar to glass and opaque parts similar to metal. The transparent parts were thought to be non-crystalline (amorphous). However, when measurement of the transparent part was actually performed, only one diffraction line was observed. In general, when measurement is done with a 0D or a 1D detector, the range in which diffraction X-rays can be detected from the sample is limited to a certain region. (This region is indicated in yellow in Fig. 1.) In this experiment, the diffraction lines, indicated with red arrows, are within the scanning range, and therefore can be detected. On the other hand, it is possible to acquire information on multiple lattice planes, as shown in Fig. 2, by using the HyPix-3000, which has a wide effective detection area, and by performing measurement while oscillation the sample. When the 2D diffraction image obtained in Fig. 2 was converted to a 2θ-I profile and qualitative analysis of the transparent part was carried out, it was possible to identify forsterite (Mg₂SiO₄) (Fig. 3). Since diffraction spots were observed, it was conjectured that the glass-like transparent part is a single crystal or is comprised of an extremely small number of crystal grains.
Fig. 1: X-ray diffraction pattern of glass-like transparent part when using a 0D or 1D detector
Fig. 2: 2D diffraction image of transparent part (with oscillation)
Fig. 3: Result of qualitative analysis of transparent part
Recommended equipment and software
- Automated Multipurpose X-ray Diffractometer SmartLab
- 2D Hybrid Pixel Array Detector HyPix-3000
The SmartLab is the most novel high-resolution diffractometer available today.
Perhaps its most novel feature is the SmartLab Guidance software,
which provides the user with an intelligent interface that guides you through the intricacies of each experiment. It is like having an expert standing by your side.
For more information click here.
21. januar 2017
Removable Chambers or All-in-One Chambers for Immunofluorescence Assays.
Classic Immunofluorescence in Removable Chambers
ibidi provides the easiest and most convenient solution for removable culture wells. These products contain a silicone gasket with chambers for individual cell culture and incubation conditions. The Immunofluorescence Chamber Family is perfect for long-term storage of samples prepared using the classic "glass slide with mounted coverslip" technique.
Immunofluorescence in All-in-One Chambers
ibidi provides a time saving and cost-effective solution for immunofluores¬cence with ibidi µ-Slides, an All-in-One chamber. Choose between the traditional open-well chambers or the channel µ-Slides. The channel slides are ideal for reducing the needed volume of expensive reagents or staining antibodies. Both formats allow cultivation, fixation, permeabilization, and staining steps to be completed in the wells or channels. After staining and mounting, the sample can be observed through the coverslip-like bottom by high-resolution microscopy.
Find more information how to optimize your immunofluorescence protocol with these application notes:
Application Note 49: Immunofluorescence Staining with the 12 Well Chamber, removable
Application Note 50: Immunofluorescence Staining with the 3 Well Chamber, removable
Application Note 45: Mounting Medium Types for Immunofluorescence Microscopy
Interested in testing how the slides work?
Order Your FREE Sample
for Your Immunofluorescence Assay.
µ-Slide 2 Well, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
µ-Slide 4 Well, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
µ-Slide 8 Well, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
µ-Slide VI 0.4, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
3 Well Chamber, removable: microscopy glass slide, sterilized
8 Well Chamber, removable: microscopy glass slide, sterilized
12 Well Chamber, removable: microscopy glass slide, sterilized
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