14. juni 2017

Advanced analysis of human T-cell subsets on the CytoFLEX

Application Information Bulletin: 13 colors on CytoFLEX

Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube based on DuraClone dry reagent technology.

 

 

 

Summary
Multi-color flow cytometry is a powerful tool to analyze the highly heterogeneous human T cell compartment.
Using the 10 color DuraClone IM T Cell Subset dry reagent kit (CD45RA-FITC, CD197-PE, CD28-ECD,
CD279-PC5.5, CD27-PC7, CD4-APC, CD8-Alexa-Fluor* 700, CD3-APC-Alexa-Fluor* 750, CD57-Pacific Blue*,
CD45-Krome Orange) plus 3 additional liquid antibodies for the violet laser (Brilliant Violet 605TM anti-human
CD95, Brilliant Violet 650TM anti-human CD25, and Brilliant Violet 785TM anti-human CD127 antibodies) we
defined a 13-color tube which allows for the identification of major peripheral T cell subsets according to
classical and more recent characterization criteria, with a minimum of sample preparation effort.

 

Download the full application here

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21. januar 2017

Immunoflourescence Assays

Removable Chambers or All-in-One Chambers for Immunofluorescence Assays.

 


 

 

 

 

 

Classic Immunofluorescence in Removable Chambers


ibidi provides the easiest and most convenient solution for removable culture wells. These products contain a silicone gasket with chambers for individual cell culture and incubation conditions. The Immunofluorescence Chamber Family is perfect for long-term storage of samples prepared using the classic "glass slide with mounted coverslip" technique.


Immunofluorescence in All-in-One Chambers

ibidi provides a time saving and cost-effective solution for immunofluores¬cence with ibidi µ-Slides, an All-in-One chamber. Choose between the traditional open-well chambers or the channel µ-Slides. The channel slides are ideal for reducing the needed volume of expensive reagents or staining antibodies.  Both formats allow cultivation, fixation, permeabilization, and staining steps to be completed in the wells or channels.  After staining and mounting, the sample can be observed through the coverslip-like bottom by high-resolution microscopy.

 

Find more information how to optimize your immunofluorescence protocol with these application notes:

Removable Chambers:
Application Note 49: Immunofluorescence Staining with the 12 Well Chamber, removable
Application Note 50: Immunofluorescence Staining with the 3 Well Chamber, removable
Application Note 45: Mounting Medium Types for Immunofluorescence Microscopy

All-in-One Chambers
Application Note 16: Immunofluorescence Staining with the µ-Slide 8 Well
Application Note 09: Immunofluorescence Staining with the µ-Slide VI 0.4

Interested in testing how the slides work? 
Order Your FREE Sample
for Your Immunofluorescence Assay.

Ordering information:
µ-Slide 2 Well, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
µ-Slide 4 Well, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
µ-Slide 8 Well, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
µ-Slide VI 0.4, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
3 Well Chamber, removable: microscopy glass slide, sterilized
8 Well Chamber, removable: microscopy glass slide, sterilized
12 Well Chamber, removable: microscopy glass slide, sterilized

 

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