23. juni 2017

Hvornår bør en centrifuge udskiftes?

Guide til centrifuger fra Lab Manager her.

Lab Manager har publiceret en film der giver et indblik i hvornår det kan være tid til at udskifte en centrifuge i et laboratorie.

Filmen kan ses herunder:

 

 

 

 

Derudover er der lavet en skreven guide omhandlende centrifuger, der dækker følgende emner:

  • Spørgsmål omkring indkøb af ny centrifuge
  • Vedligeholdelse af centrifuger
  • Ekspertråd omkring opsætning og teknikker
  • Aktuelle produkter

Guiden kan downloades her

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14. juni 2017

Advanced analysis of human T-cell subsets on the CytoFLEX

Application Information Bulletin: 13 colors on CytoFLEX

Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube based on DuraClone dry reagent technology.

 

 

 

Summary
Multi-color flow cytometry is a powerful tool to analyze the highly heterogeneous human T cell compartment.
Using the 10 color DuraClone IM T Cell Subset dry reagent kit (CD45RA-FITC, CD197-PE, CD28-ECD,
CD279-PC5.5, CD27-PC7, CD4-APC, CD8-Alexa-Fluor* 700, CD3-APC-Alexa-Fluor* 750, CD57-Pacific Blue*,
CD45-Krome Orange) plus 3 additional liquid antibodies for the violet laser (Brilliant Violet 605TM anti-human
CD95, Brilliant Violet 650TM anti-human CD25, and Brilliant Violet 785TM anti-human CD127 antibodies) we
defined a 13-color tube which allows for the identification of major peripheral T cell subsets according to
classical and more recent characterization criteria, with a minimum of sample preparation effort.

 

Download the full application here

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14. marts 2017

Lynhurtig kvalitetskontrol på foder

Kvalitetskontrol af råvarer, ingredienser, mix eller færdigvarer med Røntgen Flourescens er nu hurtigere og bedre end nogensinde.

Kvalitetskontrol af råvarer, ingredienser, mix eller færdigvarer med Røntgen Flourescens er nu hurtigere og bedre end nogensinde.

Bestemmelse af Na, Mg, P, Cl, K, Ca, Mn, Fe og mange flere, med detektions grænser fra 1 ppm - måles direkte, uden nævneværdig prøve forberedelse.

Takket være den rivende udvikling af detektorer, optik og med Rigaku's RFP-SQX software, måles alle elementer på én gang og uden kalibrering.

Læs hele "Analysis of Animal Feeds"-applikationen fra Rigaku her. 

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16. februar 2017

Fast Glycan Labeling & Analysis

Nyt revolutionerende kit fra SCIEX, der henvender sig til alle CE-brugere.

SCIEX Fast Glycan Labeling and Analysis delivers rapid glycan heterogeneity identification, capable of profiling your glycans in record time. Combining simplified sample preparation, rapid separations and automated glycan identification, you can make confident decisions quickly by running glycans up to five-times faster than HILIC.

 

 

 

 

Up to 5x Faster Than HILIC

SCIEX Fast Glycan technology enables you to rapidly perform glycan separation while retaining high resolution information. This means you can characterize more samples in less time for quicker comparability assessments across a population of samples. You can also minimize the cycle time needed to course correct processes, from development to commercial manufacturing.

Automated and Immediate Glycan Identification

The comprehensive GU based glycan reference database delivers fast separations with automated and immediate identification. Key glycan GU values are established using the SCIEX Fast Glycan assay and offer efficient identification - reducing time and minimizing errors - without the need to use technologies requiring advanced expertise.

Sample Preparation Made Easy

Based on award winning magnetic-bead technology, the SCIEX Fast Glycan assay does not require ultra-centrifugation or advanced pipetting techniques. This makes the assay suitable for manual pipetting as well as automation. The short incubation times, combined with magnetic-bead based sample clean-up, allows you to complete sample prep in an hour. Furthermore, the magnetic-bead reduces the presence of unbound dye resulting in better characterization of complex sialylated structures.

 

Click here for more information

 

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10. februar 2017

Stony-iron meteorite analysis

Analysis of a stony-iron meteorite using a HyPix-3000 multi-dimensional detector.

Introduction
A stony-iron meteorite is like a single crystal. When using 0- or 1-dimensional detectors, only a few diffraction peaks are observed. In this situation, identification of crystalline phases cannot be conducted. Therefore, a stony-iron meteorite was analyzed using the multi-dimensional detector, HyPix-3000, which has an effective detection area for the identification of crystalline phases.

 

 

Measurements and results
A stony-iron meteorite was analyzed using the HyPix-3000. Inside a stony-iron meteorite, there are transparent parts similar to glass and opaque parts similar to metal. The transparent parts were thought to be non-crystalline (amorphous). However, when measurement of the transparent part was actually performed, only one diffraction line was observed. In general, when measurement is done with a 0D or a 1D detector, the range in which diffraction X-rays can be detected from the sample is limited to a certain region. (This region is indicated in yellow in Fig. 1.) In this experiment, the diffraction lines, indicated with red arrows, are within the scanning range, and therefore can be detected. On the other hand, it is possible to acquire information on multiple lattice planes, as shown in Fig. 2, by using the HyPix-3000, which has a wide effective detection area, and by performing measurement while oscillation the sample. When the 2D diffraction image obtained in Fig. 2 was converted to a 2θ-I profile and qualitative analysis of the transparent part was carried out, it was possible to identify forsterite (Mg₂SiO₄) (Fig. 3). Since diffraction spots were observed, it was conjectured that the glass-like transparent part is a single crystal or is comprised of an extremely small number of crystal grains.

 

 

 

 

 

 

Fig. 1: X-ray diffraction pattern of glass-like transparent part when using a 0D or 1D detector

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Fig. 2: 2D diffraction image of transparent part (with oscillation)

 


Fig. 3: Result of qualitative analysis of transparent part


Recommended equipment and software

  • Automated Multipurpose X-ray Diffractometer SmartLab
  • 2D Hybrid Pixel Array Detector HyPix-3000

 

 

 

 

 

 

 

 

 

The SmartLab is the most novel high-resolution diffractometer available today.  
Perhaps its most novel feature is the SmartLab Guidance software,
which provides the user with an intelligent interface that guides you through the intricacies of each experiment. It is like having an expert standing by your side.
For more information click here.

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21. januar 2017

Immunoflourescence Assays

Removable Chambers or All-in-One Chambers for Immunofluorescence Assays.

 


 

 

 

 

 

Classic Immunofluorescence in Removable Chambers


ibidi provides the easiest and most convenient solution for removable culture wells. These products contain a silicone gasket with chambers for individual cell culture and incubation conditions. The Immunofluorescence Chamber Family is perfect for long-term storage of samples prepared using the classic "glass slide with mounted coverslip" technique.


Immunofluorescence in All-in-One Chambers

ibidi provides a time saving and cost-effective solution for immunofluores¬cence with ibidi µ-Slides, an All-in-One chamber. Choose between the traditional open-well chambers or the channel µ-Slides. The channel slides are ideal for reducing the needed volume of expensive reagents or staining antibodies.  Both formats allow cultivation, fixation, permeabilization, and staining steps to be completed in the wells or channels.  After staining and mounting, the sample can be observed through the coverslip-like bottom by high-resolution microscopy.

 

Find more information how to optimize your immunofluorescence protocol with these application notes:

Removable Chambers:
Application Note 49: Immunofluorescence Staining with the 12 Well Chamber, removable
Application Note 50: Immunofluorescence Staining with the 3 Well Chamber, removable
Application Note 45: Mounting Medium Types for Immunofluorescence Microscopy

All-in-One Chambers
Application Note 16: Immunofluorescence Staining with the µ-Slide 8 Well
Application Note 09: Immunofluorescence Staining with the µ-Slide VI 0.4

Interested in testing how the slides work? 
Order Your FREE Sample
for Your Immunofluorescence Assay.

Ordering information:
µ-Slide 2 Well, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
µ-Slide 4 Well, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
µ-Slide 8 Well, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
µ-Slide VI 0.4, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
3 Well Chamber, removable: microscopy glass slide, sterilized
8 Well Chamber, removable: microscopy glass slide, sterilized
12 Well Chamber, removable: microscopy glass slide, sterilized

 

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